The Port of Long Beach on Monday said it has placed a $35 million order for 300 heavy-duty trucks in an effort to jumpstart its Clean Trucks Program, which is set to take effect Oct. 1.

The order is part of an overall $2.2 billion program to replace all pre-1989 trucks that haul freight in the harbor.

“We want to bring as many clean trucks to Long Beach as soon as we can, and especially replacements for the 1988 and older trucks that will not be allowed to work here beginning October 1,” Richard Steinke, the port’s executive director, said in a statement.

The order caps more than a year of negotiations that will allow the landmark program to put an entire new fleet at one of the nation’s busiest container ports in an effort to reduce pollution by 80 percent.

The Port of Los Angeles is pushing ahead with a separate version of the plan, recently approved by L.A. Mayor Antonio Villaraigosa, which will require trucking companies to hire employee drivers. Long Beach’s plan will allow the companies to continue using independent contract drivers or switch to employees.

To help truckers quickly replace the old polluting vehicles, the ports are providing truckers and motor carriers with financial assistance in the form of grants or leases to buy the new clean trucks.

The 300 trucks will consist of 100 liquefied natural gas-powered trucks and 200 clean diesel-powered vehicles, the Long Beach port said.

The trucking industry has objected to the plan, saying it will drive up costs and allow unions to organize drivers. The American Trucking Association has repeatedly said it will file a lawsuit to block the program.